Pasteur Courses

Cryo-ET : Image Processing for Electron Microscopy/Tomography

The course is aimed at PhD students, post-doctoral fellows, engineers, and researchers who have some experience in (Cryo-)EM and would like to use (Cryo-)ET in the near future. Applicants who already have tilt-series for processing will be prioritized. The course aims to provide all the theoretical background needed and useful tools to render the trainees autonomous in Electron Tomography.

Practical Information

  • 1 week
  • June 21, 2027 - June 25, 2027
  • Institut Pasteur
  • English

Contacts

CryoET@pasteur.fr

Registration

  • Deadline : March 1, 2027
  • Attendees : 14

Accreditations:

  • Professional training of pasteurian staff
  • Institut Pasteur Diploma
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Description

Electron tomography combined with image processing is one of the most powerful methods for studying cellular details in 3D. In situ studies can reveal additional important information for macromolecules and their complex environments, their adopted conformations, recruitment on particular sites and interactions with other key molecules or ultrastructures. 

 
This one-week course aims to introduce basic concepts in image analysis and practical experience in tomographic reconstructions for different needs: processing of low-dose tilt series with and without fiducials for 3D volume annotation or subtomogram averaging. Introduction to tomography, frames alignments, tomogram reconstruction, denoising, volume annotation and particle picking for subtomogram analysis will be covered. 

 Interested in CryoEM? Lady Pipette, a PhD student at the Pasteur Institute, explains it with music!

 

Download the image

Directors

GAZI Anastazia - Institut Pasteur
SARTORI-RUPP Anna - Institut Pasteur

Members of the course committee

CHERIET Samia - Institut Pasteur, Paris
GAZI Anastazia - Institut Pasteur
SARTORI-RUPP Anna - Institut Pasteur